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KMID : 1144820180240040365
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대한의생명과학회지
2018 Volume.24 No. 4 p.365 ~ p.371
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Anti-Oxidative and Anti-Neuroinflammatory Effect of Ethanol Extracts from Walnuts's (Juglans regia L.) Shell
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Kang Hyun
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Abstract
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In this study, antioxidant and anti-neuroinflammatory of ethanol extracts from walnuts's (Juglans regia L.) shell were investigated in vitro. Radical-scavenging activities of the walnuts's shell ethanol extracts (WSE) were examined by using ABTS radicals and α,α-diphenyl-β-picrylhydrazyl (DPPH) radicals assay. In the ABTS and DPPH radical scavenging activity, RC50 of WSE were measured as 15.74 and 40.13 μg/mL, respectively. Also, to evaluate the anti-neuroinflammatory effects of WSE in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells. The production of proinflammatory cytokines NO were examined by LPS in BV-2 cell. BV-2 cells activated with LPS were treated with various doses (10, 25, 50, 100 μg/mL) of WSE. Supernatants were analyzed for the production of NO using Griess reagent. WSE up to 10 μg/mL still required to inhibit NO induced by LPS. These results showed that walnuts's (Juglans regia L.) shell can be used as an easily accessible source of natural anti-neuroinflammatory and natural antioxidants.
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KEYWORD
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Walnuts, Anti-inflammatory activity, ABTS, Microglial cells, NO
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